In Situ Hybridization Protocols Hardcover - 2005
by Ian A. Darby (Editor); Tim D. Hewitson (Editor)
From the rear cover
In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully.
Details
- Title In Situ Hybridization Protocols
- Author Ian A. Darby (Editor); Tim D. Hewitson (Editor)
- Binding Hardcover
- Edition U. S. EDITION
- Pages 270
- Volumes 1
- Language ENG
- Publisher Humana
- Date 2005-12-15
- Illustrated Yes
- ISBN 9781588294029 / 1588294021
- Weight 1.44 lbs (0.65 kg)
- Dimensions 9.12 x 6.3 x 0.97 in (23.16 x 16.00 x 2.46 cm)
- Library of Congress subjects In situ hybridization - Laboratory manuals, In Situ Hybridization
- Library of Congress Catalog Number 2005015572
- Dewey Decimal Code 572.84
Media reviews
Citations
- Scitech Book News, 06/01/2006, Page 42
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